The pGEM®- T Easy Vector Systems are convenient systems to clone PCR products generated by certain thermostable polymerases. These polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments.
pGEM®- T Easy Parental vector for TA cloning of PCR products. The insertion site is flanked by BstZI, EcoRI, and NotI sites.
pGEM®-T Easy Vector Systems – Promega, pGEM®-T and pGEM®-T Easy Vector Systems Protocol, Addgene: Vector Database – pGEM-T Easy Vector, pGEM -T and pGEM -T Easy Vector Systems – Promega, T -Overhangs for Easy PCR Cloning: The pGEM ®- T and pGEM -T Easy Vectors are linearized vectors with a single 3´-terminal thymidine at both ends. The T -overhangs at the insertion site greatly improve the efficiency of ligation of PCR products by preventing recircularization of the vector and providing a compatible overhang for PCR products, The only difference between pGEM – T and pGEM-T Easy is in the multiple cloning site (MCS). The MCS of the pGEM-T Easy Vector contains sequences on either side of the insert that are recognized by the restriction enzymes Not I and EcoR I. This allows the insert DNA to be removed with a single restriction digest using either of these enzymes.
The pGEM®- T Easy Vector Systems are convenient systems to clone PCR products. They offer all of the advantages of the pGEM ®- T Vector Systems with the added convenience of recognition sites for EcoRI and NotI flanking the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion.
The pGEM ®- T and pGEM ®-T Easy Vector Systems are convenient systems for the cloning of PCR products. The vectors are prepared by cutting the pGEM ®-5Zf(+) and pGEM ®-T Easy Vectors, respectively, with EcoR V and adding a 3´ terminal thymidine to both ends. These single 3´- T overhangs at the insertion site greatly improve the efficiency …
pGEM®- T Easy Vector System????? pGEM ®- T Vector System???????????????????????EcoRI?NotI?????????????????1???NotI?EcoRI????BstZI???????????????????????????DNA???????????????, The pGEM ®- T Vector is derived from the pGEM ®-5Zf(+) Vector (GenBank® Accession No. X65308). The pGEM ®- T Vector was created by linearizing the pGEM ®-5Zf(+) Vector with EcoRV at base 51 and adding a T to both 3´-ends. The position of the T is indicated by * in the pGEM ®- T Vector Sequence (.txt).
There is no GenBank® Accession Number for the pGEM®- T Easy Vector. The parent vector is linearized at the position indicated by * in this pGEM®- T Easy Vector Sequence and a T is added at each end.
