To calculate the Rf value for each pigment use the following formula: Rf Value = distance travelled by the pigment distance travelled by the solvent R f Value table for the solvent used (90% propanol, 10% ether) Pigment R f value Carotene 0.94 Xanthophyll 0.89 Chlorophyll A 0.46 Chlorophyll B 0.22.
TLC Plate Rf Value (± 0.05 cm) Chlorophyll Pigment Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Chlorophyll a 0.24 – – – – – – – Chlorophyll b 0.20 0.69 – – – – – – Carotene 0.31 0.96 – – – – – – Xanthophyll 0.90 0.58 – – – – – – * The uncertainties are derived from the uncertainty of a ruler* Graph Graph 1: Showing relationship between pigments in …
You can obtain Rf values for each of your pigment lines using the equation in Figure 1. The literature reports the following Rf values for each component: Rf = 0.16 for xanthophyll, Rf = 0.32 for chlorophyll b, Rf = 0.44 for chlorophyll a, and Rf = 0.95 for ?- carotene.
3/30/2007 · The pigments and their Rf’s were: carotene – 0 .98 chlorophyll a – 0.59 chlorophyll b – 0.42 pheophytin – 0.81 xanthophyll 1 – 0.28 xanthophyll 2 – 0.15 The color of the bands can be a general guide to identify the pigments. Carotene is orange. Chorophylls are green. Chlorophyll a is a blue-green. Chlorophyll b is a yellow-green. Xanthophylls are yellow.
Standard Rf Values – Biology (Photosynthesis) | Physics Forums, Colour, chlorophyll and chromatography | www.scienceinschool.org, Plant Pigment Paper Chromatography – TTU, Colour, chlorophyll and chromatography | www.scienceinschool.org, Plant Pigment Chromatography Students will isolate and identify photosynthetic pigments in spinach leaves. Students will calculate Rf values of photosynthetic pigments and graph the absorption spectrum for each pigment . Introduction As primary producers in the food chain with some bacteria and algae, plants, The retention ( Rf ) value for each one of the pigments and the concentration of chlorophyll a and b were calculated, and all of the results were put in tables. The ratio of chlorophyll a to chlorophyll b was then also calculated and the result was 2.6:1, which is quite close to the result expected .
carotene – 0.98 chlorophyll a – 0.59 chlorophyll b – 0.42 pheophytin – 0.81 xanthophyll 1 – 0.28 xanthophyll 2 – 0.15 everything is in percentages, Pigment Rf value range Colour Relative position Lutein 0.22-0.28 Yellow Below, or almost at the same level of, the highest green Violaxanthin 0.13-0.19 Yellow Below, or almost at the same level of, the highest green Neoxanthin 0.04-0.09 Yellow.
Rf Values Of Pigments In Paper Chromatography
